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. 2018 Aug 24;5:77. doi: 10.3389/fmolb.2018.00077

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Copyright © 2018 Di Mise, Ranieri, Centrone, Venneri, Tamma, Valenti and Valenti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reduced mitochondrial calcium levels in human ciPTEC-PC1KD: effect of CaSR positive allosteric modulator NPS-R568. Evaluation of mitochondrial Ca²⁺ with mitochondria-targeted cameleon (4mtD3cpV) FRET probe. Histogram compares changes in normalized FRET (netFRET) ratio between ciPTECwt and ciPTEC-PC1KD, at basal conditions or treated with NPS-R568. At rest, ciPTEC-PC1KD presented a significant lower mitochondrial calcium content with respect to ciPTECwt (**P < 0.01). CaSR stimulation with NPS-R568 10 μM induced a significant increase in calcium levels in ciPTEC-PC1KD, restoring levels close to ciPTECwt at basal conditions. All data were analyzed by One-way ANOVA followed by Newman–Keuls multiple comparisons test and expressed as means ± SEM (*P < 0.01 ciPTEC-PC1KD+NPS-R568 vs. ciPTEC-PC1KD Basal; ^#P < 0.001 ciPTECwt+NPS-R568 vs. ciPTECwt Basal).