Table 2.

Clades and subclades used for MLST analysis.

Clade	Species included	No. of species	GC (mol%)a	MLST (%)b	AAI (%)c	Phi test (P value)d	Habitate
Anguillarum	V. anguillarum, V. aestuarianus and V. ordalii	3	43–46	95.5	99	87.0–98.3 95.8–99.8	Brackish water, seawater and fish
Cholerae	V. cholerae, V. mimicus, V. furnissii, V. fluvialis, V. metschnikovii and V. cincinnatiensis	6	44–50	87	96.3	83.4–94.4 93.5–99.8	Brackish water, seawater
Coralliilyticus	V. coralliilyticus and V. neptunis	2	45–46	97	99.7	94.2 99.5	Seawater, bivalves and rotifers
Harveyi	V. harveyi, V. campbellii, V. parahaemolyticus, V. alginolyticus, V. mytili, V. natriegens, and V. rotiferianus	7	42–48	91.3	97.8	86.7–96.0 96.9–99.9	Seawater, salt marsh mud and marine animals
Orientalis	V. orientalis, V. hepatarius, V. brasiliensis, V. sinaloensis, V. tubiashii and BA55 strain	6	43–46	95.9	99.2	100	Brackish water and seawater
Splendidus	V. splendidus, V. crassostrea, V. cyclitrophicus, V. chagasii, V. fortis, V. kanaloaei, V. lentus, V. gigantis, V. pelagius, V. tasmaniensis and V. pomeroyi	11	39–47	98.2	99.8	88.8–93.7 97.1–98.9	Seawater and marine animals
Vulnificus	V. vulnificus and V. navarrensis	2	45–48	93.6	98.8		Sewage, seawater and oyster
Damselae	P. damselae	1	42				Seawater and fish

^aGC content calculated based on six genes.

^bMLST percentage of concatenate similarity between sequences.

^cAAI Raw average amino acid identity (10).

^dPhi test. The phi test was conducted for clades that included at least four species.

^eHabitat described for Sawabe et al.¹³.