Fig. 4.

CXCR4 receptor regulates DNA-damage associated inflammation. For all experiments, media from treated cells (as indicated), was used for sandwich ELISA for determining levels of IL8 or IL6 pg/ml secreted per 10³ cells, represented as fold change over control untreated cells. a Effect of activation of CXCR4-CXCL12 signaling during DNA damage on IL8 and IL6 cytokine secretion (n > 6). b Effect of CXCR4 inhibition (AMD treatment) on IL6 production from senescent cells post-CXCL12 stimulation. Cells were treated with various compounds as indicated and IL6 levels in supernatant were analysed (n = 3). c Effect of inhibition of Gαi by PTx treatment. Comparison of IL8 levels between HeLa cells treated with BrdU; BrdU + CXCL12 or BrdU + CXCL12 in presence of pertussis toxin (PTx) (n = 3). d Effect of inhibition of Gαi by PTx treatment on MRC5 cells. Comparison of IL8 levels in MRC5, primary cells treated with BrdU; BrdU + CXCL12 and BrdU + CXCL12 in presence of PTx (n = 3). For all experiments, results are represented as mean ± s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001 (Student’s t-test)