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. 2018 May 18;37(35):4871–4886. doi: 10.1038/s41388-018-0302-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — pancEts-1 is a lncRNA associated with poor survival of gastric cancer. a Scheme indicating the existence of pancEts-1 transcribed upstream the Ets-1 promoter region. b RNA fluorescence in situ hybridization images showing the nuclear and cytoplasmic localization of pancEts-1 in MKN-45 cells using a 138-bp antisense probe (red), with the nuclei staining by DAPI (blue). Sense probe and antisense probe with RNase A (20 μg) treatment were used as negative controls. Scale bars: 10 μm. c Real-time qRT-PCR assay revealing the pancEts-1 transcript levels (normalized to β-actin) in normal gastric mucosa (n = 30) and cultured gastric cancer cell lines (mean ± SD, n = 5). d Real-time qRT-PCR assay indicating the differential expression of pancEts-1 transcript (normalized to β-actin) in normal gastric mucosa (n = 30) and gastric cancer tissues (n = 81). e, f Real-time qRT-PCR assay showing the pancEts-1 transcript levels (normalized to β-actin) in gastric cancer tissues with differential status of metastasis (e) or Ets-1 immunostaining (f). g The positive correlation between pancEts-1 and Ets-1 transcript levels in gastric cancer tissues (n = 81). h Kaplan–Meier curves indicating overall survival of 81 gastric cancer patients (cutoff value = 2.833) and overall (OS) and first progression (FP) survival of those derived from Kaplan-Meier plotter with low or high pancEts-1 expression (cutoff values = 14.0 and 14.0). Student’s t test compared gene expression levels in c–f. Pearson’s correlation coefficient analysis in g. Log-rank test for survival comparison in h