Fig. 5.

pancEts-1 harbors oncogenic properties through its interplay with NONO. a, b Western blot (a) and real-time qRT-PCR (b) assays revealing the differential protein and transcript levels of Ets-1 (normalized to β-actin) in gastric cancer cells stably transfected with mock, pancEts-1, NONO, sh-Scb, sh-NONO, or sh-pancEts-1 (mean ± SD, n = 4). c d Representative images (left) and quantification (right) of soft agar (c) and transwell matrigel invasion (d) assays indicating the anchorage-independent growth and invasion capability of gastric cancer cells stably transfected with mock, pancEts-1, NONO, sh-Scb, sh-NONO #2, or sh-pancEts-1 #2 (mean ± SD, n = 6). e In vivo growth curve (left), representative images (middle upper) and tumor weight (middle lower) at the end points of xenografts in athymic nude mice formed by hypodermic injection of NCI-N87 cells stably transfected with mock, pancEts-1, sh-Scb, and sh-NONO #2 (n = 5 for each group). Quantification of lung metastatic colonies (middle) and Kaplan–Meier curves (right) of nude mice treated with tail vein injection of NCI-N87 cells stably transfected with mock or pancEts-1, and those co-transfected with sh-Scb or sh-NONO #2 (n = 5 for each group). Student’s t test and analysis of variance analyzed the difference in b–e Log-rank test for survival comparison in e. *P < 0.01 vs. mock + sh-Scb. NS, not significant