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. 2018 May 18;37(35):4871–4886. doi: 10.1038/s41388-018-0302-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — The expression of NONO, ERG, or Ets-1 is associated with poor outcome of gastric cancer patients. a Representative immunohistochemical staining images showing the nuclear and cytoplasmic expression pattern of NONO, ERG, and Ets-1 in normal gastric mucosa, precancerous gastric mucosa, and tumor cells of gastric cancer specimens (arrowheads, brown). Scale bars: 100 μm. b Western blot assay indicating the differential levels of NONO, ERG, and Ets-1 in normal gastric mucosa (N, n = 30), precancerous tissues (P, n = 81), and gastric cancer tissues (c, n = 81). c Real-time qRT-PCR showing the expression levels (normalized to β-actin) of NONO and ERG in normal gastric mucosa (n = 30), gastric cancer tissues (n = 81), and gastric cancer specimens with (n = 22) or without metastasis (n = 59). d Real-time qRT-PCR assay revealing the NONO and ERG transcript levels (normalized to β-actin) in normal gastric mucosa (n = 30) and cultured gastric cancer cell lines (mean ± SD, n = 5). e The positive correlation between ERG and Ets-1 transcript levels in gastric cancer tissues (n = 81). f Kaplan–Meier curves indicating overall (OS) and first progression (FP) survival of gastric cancer patients derived from Kaplan-Meier plotter with low or high expression of NONO (cutoff values = 7825.0 and 7797.0), ERG (cutoff values = 225.0 and 126.0), or Ets-1 (cutoff values = 126.0 and 89.0). g The mechanisms underlying pancEts-1-drived progression of gastric cancer: as a novel lncRNA, pancEts-1 interacts with NONO to facilitate the physical interaction between NONO and ERG, resulting in transactivation of ERG and transcription of Ets-1 that are associated with gastric cancer progression. Student’s t test analyzed the difference in c and d. Pearson’s correlation coefficient analysis in e. Log-rank test for survival comparison in f