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. 2001 Nov 6;98(24):14090–14095. doi: 10.1073/pnas.221467298

Figure 5.

Figure 5

Recovery of the HB-19 complexed with nucleolin from lymphoid organs. Rats were intravenously injected with 1 mg of either the biotin-labeled HB-19 or its corresponding control peptide, the biotin-labeled CP-51. At 45 min postinjection, tissue extracts were prepared as described in Materials and Methods. The HB-19 (A) or CP-51 (B) bound proteins were assayed by dot-immunoblotting using the antinucleolin polyclonal antibody. The HB-19 bound proteins were also analyzed by immunoblotting after SDS/PAGE, using mAb D3 (C). A single band was identified by the antinucleolin antibody. C shows a section of the immunoblot (10% gel) at the region of the nucleolin migration (95 kDa).