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. 2018 Aug 30;9(9):896. doi: 10.1038/s41419-018-0903-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Protein levels of ALDH1A1 and BCRP were determined in the parental A2780 cell line and doxorubicin-resistant A2780 cell line (A2780DR) by using western blot analysis. b ALDH1 activity was determined using the Aldefluor assay system in A2780 and A2780DR cells. ALDH1 inhibitor DEAB-treated cells were used as a negative control. Cell population with Aldefluor-derived fluorescence intensity was analyzed using flow cytometry. Similar results were obtained in 3–4 independent experiments. c Protein levels of ALDH1A1 were determined in ALDH-negative (ALDH−) and ALDH-positive (ALDH+) cell populations isolated from A2780DR. d Transcript levels for ALDH1A1 in ALDH− and ALDH+ cells using RT-PCR analysis. Values represent the mean ± standard deviation (SD) of three experiments. ^aP < 0.05 compared to the ALDH-L group. e ALDH enzymatic activity was assessed using the Aldefluor assay system in the established ALDH1-low A2780DR cell line (ALDH-L) and ALDH1-high A2780DR cell line (ALDH-H). Cell populations with Aldefluor-derived fluorescence intensity were analyzed using flow cytometry. Similar blots were obtained in three independent experiments (a and c)