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. 2018 Aug 30;9(9):896. doi: 10.1038/s41419-018-0903-4

Fig. 7. ATRA treatment reduces CSC-like properties in ALDH-H cells, but not in ALDH-L cells.

Fig. 7

a, b Cell viability after incubation with doxorubicin (Dox) for 24 h in ALDH-H (a) and ALDH-L cells (b). Values represent the mean ± SD from 8 to 10 sampled wells. aP < 0.05 compared to the doxorubicin-treated group. c Soft agar colony formation was assessed in ALDH-L and ALDH-H cells after ATRA treatment (10 μM, 24 h). Values represent the mean ± SD from three experiments. aP < 0.05 compared to vehicle-treated ALDH-L group, bP < 0.05 compared to vehicle-treated ALDH-H group. d Sphere formation capacity was assessed after 3 days of sphere culture of ALDH-L and ALDH-H cells. Number of spheres (over 100 μm diameter size) was quantified. Values represent the mean ± SD from three experiments. aP < 0.05 compared to vehicle-treated ALDH-L group, bP < 0.05 compared to vehicle-treated ALDH-H group. e Sphere (over 100 μm diameter) number was quantified in sc and iNRF2 ALDH-H cells following ATRA treatment. Values represent the mean ± SD from three experiments. aP < 0.05 compared to each vehicle-treated group. f Protein levels of NRF2, BCRP, MDR1, and NANOG were measured in sc and NRF2-silenced ALDH-H cells following ATRA treatment. Similar blot were obtained in three independent experiments