Fig. 1. CD59 expression level is positively correlated with radioresistance in ESCC.

a CD59 levels in radiosensitive specimens (left, n = 12) were significantly downregulated compared with those in radioresistant specimens (right, n = 12). All specimens were collected before radiotherapy from ESCC patients who received radiotherapy afterward. b Quantitative results from (a). c, d The colony formation ability of the radioresistant cells was significantly greater than that of the original cells after irradiation. The representative images in (c) and the quantitative results in (d). e CD59 was significantly upregulated in the three radioresistant cells compared with the expression in the corresponding original cells. f The comparison of CD59 expression levels in four original ESCC cell lines. g The dynamic growth of four ESCC cell lines with 5 Gy or without irradiation treatment (UT). KYSE510, KYSE180, or Eca109 vs TE1 cells. h, i The colony formation ability of four ESCC cell lines with or without irradiation. The representative images in h and the quantitative results in (i). RR: radioresistant cells to a total of 25 Gy (5 Gy 5 times). Data represent the mean ± SD, n = 3 unless otherwise indicated, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001