Fig. 5. Src inhibition potentiates the susceptibility of ESCC to irradiation.

a, b Saracatinib significantly increased the formation of γH2AX foci in Eca109 cells at 12 h after ionizing radiation. The representative images for γH2AX foci in (a) and the quantitative results in (b) for measuring the percentage of γH2AX-positive ( > 10 foci/cell) cells. Scale bar, 25 μm. c, d Saracatinib impaired the colony formation ability of Eca109 cells after irradiation. The representative images in (c) and the quantitative results in (d). e, f Saracatinib enhanced the cellular senescence induced by irradiation. The representative images in (e) and the quantitative results in (f). Cellular senescence was detected by SA-β-gal staining. Data represent the mean ± SD, n = 3, *P < 0.05, **P < 0.01 and ****P < 0.0001