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. 2018 Aug 30;8:13106. doi: 10.1038/s41598-018-30989-3

Figure 6.

Figure 6

High level of miR-10b “hijacks” linifanib and prevents it from inhibiting its kinase targets in cancers. (A) Schematic representation of dose-dependent experiments (left panel) and western blots (right panel) showing the expression of p-VEGFR and p-PDGFR increased, but the expression of total VEGFR and PDGFR as well as HOXD10 and PTEN decreased after transfection with increasing doses of pre-miR-10b (0, 5, 20, 80 nM) and treatment of 10 μM linifanib for 24 hrs. On the left side of the blots, proteins names and the normalization methods are presented. (B) Schematic representation of rescue experiments (left panel) and western blots (right panel) showing the p-VEGFR and p-PDGFR decrease mediated by increasing doses of linifanib (0, 5, 10, 15 µM) could be rescued when transferring increasing doses of pre-miR-10b (0, 5, 20, 80 nM) in HepG2 cells. On the left side of the blots, proteins names and the normalization methods are presented.