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. 2018 Aug 30;9:3520. doi: 10.1038/s41467-018-06034-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Transcriptional regulation of mutations at interfacial LBD residues. a Sites of LBD mutation (I326, Y328, P406, and L409), highlighted in black circles. b–c Transient transfection reporter activity of the hERα and the Gal4-DBD/hERα-LBD fusion protein, using an ERE-TK-Luc (b) or a Gal4-TK-Luc reporter construct (c), respectively. Triplicates were carried out and standard deviations were indicated