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. 2018 Aug 30;9(9):888. doi: 10.1038/s41419-018-0882-5

Fig. 2. Downregulation of SNHG5 induces apoptosis and promotes proliferation, migration and invasion in HCC lines. Data are shown as the means ± s.d. *P < 0.05; **P < 0.01.

Fig. 2

a qRT-PCR analysis of SNHG5 expression following transfected HCC cells with three different SNHG5-siRNAs, **P < 0.01. b MTT assays showing that silencing of SNHG5 inhibited the proliferation of HCC cells. *P < 0.05. c SNHG5 knockdown caused a decrease in the clonogenic survival of HCC cells. *P < 0.05; **P < 0.01. d, e FACS analysis showed downregulation of SNHG5 induces apoptosis and represses cell cycle progression in HCC cells. P < 0.05; **P < 0.01. f Common cell cycle-related and apoptosis-related proteins expression levels detected by western blot analysis following SNHG5 silencing. g, h Representative images of wound healing assays after SNHG5 silencing. *P < 0.05; **P < 0.01. i, j Transwell assays showed SNHG5 knockdown reduced the migration and invasion of HCC cells. (scale bars = 50 mm). **P < 0.01. k Expression levels of MMP-2 and MMP-9 after SNHG5 knockdown were analyzed by western blot