Fig. 7. SNHG5 induced EMT by competitively binding miR-26a-5p and activating Wnt/β-catenin pathway.

a qRT–PCR analysis of GSK3β mRNA expression after SNHG5 knockdown. **P < 0.01. b Pearson’s correlation analysis of the relationship between SNHG5 and GSK3β mRNA expression levels in HCC tissues. c Western blot analysis of the key components of Wnt/β-catenin pathway protein expression following SNHG5 knockdown or co-transfection with shRNA and miR-26a-5p inhibitor. d HepG2 cells adopted EMT-related cell morphology in response to SNHG5 depletion. e, f Expression levels of EMT markers in HCC cells after transfected with shRNA- SNHG5 or co-transfection with shRNA and miR-26a-5p inhibitor were analyzed by western blot and quantitative RT-PCR. *P < 0.05; **P < 0.01. g Expression levels of EMT markers in HCC cells after SNHG5 knockdown or co-transfection with shRNA and miR-26a-5p inhibitor were analyzed by immunofluorescence