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. 2018 Aug 24;9:1906. doi: 10.3389/fimmu.2018.01906

Figure 2.

Figure 2

Knockdown of arachidonate 15-lipoxygenase (ALOX15) and arachidonate 15-lipoxygenase, type B (ALOX15B) inhibits sterol regulatory element binding protein (SREBP)-2 activation. (A) Representative Western analysis of SREBP-2 and mature N-terminal SREBP-2 (mSREBP-2) in macrophages transfected with control, ALOX15, or ALOX15B siRNAs 24 h prior to treatment with interleukin-4 (IL-4) for 48 h. (B–D) mRNA expression of (B,C) low density lipoprotein receptor (LDLR), mevalonate kinase (MVK), acetoacetyl-CoA synthetase (AACS), acetyl-CoA acetyltransferase 2 (ACAT2), insulin-induced gene 1 (INSIG1) (D) and SREBP-1c, apolipoprotein E (APOE), liver X receptor (LXR)α, myosin regulatory light chain interacting protein (MYLIP), ABCG1, ABCA1 in macrophages transfected with control and ALOX15 (B,D) or ALOX15B (C,D) siRNAs 24 h prior to treatment with IL-4 for 48 h. P-values were calculated using two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001. Data are mean values ± SE of at least three independent experiments.