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. 2018 Aug 24;9:1906. doi: 10.3389/fimmu.2018.01906

Figure 5.

Figure 5

Chemokine modulation by 15-LOX isoforms in interleukin-4 (IL-4)-stimulated macrophages. (A) mRNA expression of CCL8, CCL13, CCL17, CCL18, CCL22, and CCL24 in primary human macrophages treated with 20 ng/ml IL-4 for 48 h. (B,C) Chemokine mRNA expression in macrophages transfected with control and arachidonate 15-lipoxygenase (ALOX15) (B) or arachidonate 15-lipoxygenase, type B (ALOX15B) (C) siRNAs 24 h prior to treatment with IL-4 for 48 h. (D) CCL17 protein concentrations in culture medium of macrophages transfected with control, ALOX15, or ALOX15B siRNAs 24 h prior to treatment with IL-4 for 48 h. (E,F) CCL17 mRNA expression (E) and protein concentrations (F) from macrophages treated with either ML351 (10 µM) or PD146176 (10 µM) for the final 24 h post IL-4 stimulation. P-values were calculated using one-way ANOVA with Bonferroni post hoc means comparisons with significance level set at 0.05. *P < 0.05, **P < 0.01, ***P < 0.001. Data are mean values ± SE of at least three independent experiments.