Figure 5.

Silencing of SUMO-1 expression exacerbates α-syn aggregation in METH-treated cells. First, SH-SY5Y cells were transfected with siNC, siSUMO-1#1 or siSUMO-1#2 (A). Then, SH-SY5Y cells (B–D,B1–D1) and primary cultured neurons (E,F,E1,F1) were transfected with siNC or siSUMO-1 and LV-NC or LV-SUMO-1-RNAi, respectively and the cells were treated with or without METH. The cells were immunoprecipitated with an anti-SUMO-1 antibody, followed by western blot with an anti-α-syn antibody. Cells were immunoprecipitated with IgG were used as a negative control. Western blot (A–F) and quantitative analyses (A1–F1) were performed to evaluate the expression of SUMO-1 and the expression, aggregation and SUMOylation of α-syn. β-Actin was used as a loading control. *p < 0.05 compared with the NC group. ^#p < 0.05 compared with the METH-treated NC group. The data were analyzed using one-way ANOVA followed by LSD post hoc analyses.