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. 2018 Aug 24;12:262. doi: 10.3389/fncel.2018.00262

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Copyright © 2018 Zhu, Qiao, Chen, Sun, Hui, Lian, Xie, Ding, Meng, Zhu and Qiu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impaired SUMOylation of α-syn accelerates α-syn aggregation induced by METH in SH-SY5Y cells. SH-SY5Y cells stably expressing GFP, WT α-syn or α-syn-2KR were treated with or without 2.0 mM METH for 24 h. (A–C,A1–C1) In addition, UBC9 was overexpressed in SH-SY5Y cells stably expressing WT α-syn and α-syn-2KR (D,E,D1,E1). Western blot (A–E) and quantitative analyses (A1–E1) were performed to evaluate the efficiency of α-syn expression, and the expression of SUMO-1 and UBC9, and the expression, aggregation and SUMOylation of α-syn. β-Actin was used as a loading control. *p < 0.05 compared with the LV-GFP group. **p < 0.05 compared with SH-SY5Y cells stably expressing WT α-syn. ***p < 0.05 compared with SH-SY5Y cells stably expressing α-syn-2KR. ^#p < 0.05 vs. METH-treated SH-SY5Y cells stably expressing WT α-syn. The data were analyzed using one-way ANOVA followed by LSD post hoc analyses.