Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 24;12:262. doi: 10.3389/fncel.2018.00262

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Zhu, Qiao, Chen, Sun, Hui, Lian, Xie, Ding, Meng, Zhu and Qiu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Impaired SUMOylation of α-syn hinders α-syn degradation through the ubiquitin-proteasome system (UPS) and the autophagy-lysosome pathway (ALP). SH-SY5Y cells were exposed to METH in a dose-dependent manner for 24 h (A,A1–A5). SH-SY5Y cells stably expressing GFP, WT α-syn or α-syn-2KR were treated with or without 2.0 mM METH for 24 h (B,B1–B5). Western blot (A,B) and quantitative analyses (A1–A5,B1–B5) were performed to evaluate the expression of UbE1 and Ub, which are involved in the UPS, and of LC3-II, P-62 and Lysosomal associated membrane protein-2 (LAMP-2), which are involved in the ALP. β-Actin was used as a loading control. *p < 0.05 compared with the control or LV-GFP group. ^#p < 0.05 compared with the METH-treated SH-SY5Y cells stably expressing WT α-syn. Data were analyzed using one-way ANOVA followed by LSD post hoc analyses.