Figure 2.

Muscarinic agonist-mediated increase in [³H]dopamine release in striatal slices from M5R^−/− mice and their wild-type littermates. Striatal slices that had been preincubated with [³H]dopamine were depolarized with 20 mM KCl, and the resulting [³H]dopamine outflow was quantitated in the absence and the presence of the indicated concentrations of oxotremorine. Data are expressed as percent increase in [³H]dopamine release above control levels (no oxotremorine). Basal [³H]dopamine outflow did not differ between wild-type and M5R^−/− mice. Each data point represents the mean ± SEM of 9–11 independent experiments (mice). *, P < 0.05 (vs. wild type; Student's t test).