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Primers used for genotyping nitrilase mutants.
| Mutant | Primer name | Primer sequence | Target |
|---|---|---|---|
| nit1-3 | nit1-3_ko_FOR | TGCCGTTTGAAACATCTAGG | NIT1 |
| nit1-3_ko_REV | GAGTAATGTCCAACCGAATCG | NIT1 | |
| nit2 | SAIL_681_H09_FOR | CTCCCGCCACTCTAGGTAATC | NIT2 |
| SAIL_681_H09_REV | AACCATCAGCAGTAGGTGCAC | NIT2 | |
| LB3_SAIL | TAGCATCTGAATTTCATAACCAATCTCGATACAC | SAIL T-DNA | |
| nit4 | SALK016289_NIT4_F | TATGAAAGGCCCAGTGACTTG | NIT4 |
| SALK016289_NIT4_R | CACTTCAGGCCTCGAGTAATG | NIT4 | |
| LBa1 | TGGTTCACGTAGTGGGCCATCG | SALK T-DNA |
