Figure 2.

Cytokine expression by ILC in ear skin and ear draining lymph nodes during the elicitation phase of CHS. Cytokine production of all ILC subsets in the (a) ear skin and (b) ear draining lymph nodes at 48 hours after antigen challenge in CHS and challenge-only mice compared with naïve mice. (c) ICOS and CD25 expression of ILC2 isolated from ear skin (c, left graphs) and ear draining lymph nodes (c, right graphs) at 24 hours after allergen challenge in CHS and challenge-only mice. Values are shown as absolute cell numbers per 50 mg ear skin and per total ear draining lymph node, respectively. Data are shown as mean ± standard error of the mean, pooled data of three independent experiments with n ≥ 5 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. EOMES^Gfp RORγt-fm mice were used for these experiments. CHO, challenge only; CHS, contact hypersensitivity; EOMES, eomesodermin; ICOS, inducible T-cell costimulator; ILC, innate lymphoid cell; MFI, mean fluorescence intensity; NCR, natural cytotoxicity triggering receptor; NK, natural killer; ns, not significant; TNF, tumor necrosis factor.