Fig. 5.

Inhibition of protein SUMOylation in U2OS cells. 5A. U2OS were grown on 0.2% gelatin-coated coverslips. 15 min or 1 h of 100 µM GA treatment increased the mean number of talin containing FAs significantly (n = 3, mean ± SEM, GA 15 min: p < 0.0001***, GA 1 h: p = 0.0026**, one-way ANOVA with post-hoc test, 92 or 71 cells were counted for the control or GA 15 min treatment; 68 or 91 cells were counted for the control or GA 1 h treatment). 5B. Talin was a substrate for SUMOylation in U2OS cells (n = 4, individual replicated experiment). 5C. HA-SUMO-2 IP pulldown indicated that talin was SUMOylated (n = 3, individual replicated experiment).