Fig.1.

The chimeric protein of CI-M6PR failed to target to the trans-Golgi network (TGN). A: Schematic representation of different CI-M6PR truncated or chimeric proteins used in this study. Tag is attached to the N-terminus of each construct to avoid the possibility of disturbing the sorting signals in its C-terminus. Sequences from different proteins are color coded. TMD: transmembrane domain. B–E: The co-localization of TGN46 with Myc-hCI-M6PR-FL (B), HA-CD8-hCI-M6PR-C (C), HA-CD8-bCI-M6PR-C (D), and HA-Tac-hCI-M6PR-C (E) was examined by double immunofluorescent staining in HeLa Swiss cells transiently transfected with specific constructs. Scale bar, 10 μm.