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. 2018 Jun 20;32(4):245–256. doi: 10.7555/JBR.32.20180044

Fig.3.

Fig.3

The HA-tagged hCI-M6PR showed similar membrane trafficking events as that of full-length CI-M6PR. A: HA-hCI-M6PR-tail was transiently transfected in HeLa Swiss cells, followed by double immunofluorescent staining using anti-HA antibody and CI-M6PR specific antibody CD222 (upper panel). Both HA-hCI-M6PR-tail and Myc-hCI-M6PR-FL were transiently transfected in HeLa Swiss cells, followed double immunofluorescent staining in HeLa Swiss cells using anti-HA antibody and anti-Myc antibody (lower panel). Scale bar, 10 μm. B: HA-hCI-M6PR-tail was transiently transfected in HeLa Swiss cells, followed by double immunofluorescent staining using anti-HA antibody and antibodies for different subcellular organelle markers EEA1, Golgin97, TGN46, and LAMP1. Scale bar, 10 μm.