Figure 4. Glycosyltransferase activity.
Single sugar transfer activities to GlcNAc of crude cell extracts were determined by incubating 10–40 µg lysate protein with 0.125 µCi of UDP-[3H]GlcNAc and 45 µg of [GlcA-GlcNAc]n oligosaccharide acceptors. Labeled oligosaccharide products were isolated by gel chromatography and quantitated by scintillation counting. The error bars represent the mean ± S.D. from three independent experiments, each experiment performed at two different protein concentrations: *P<0.05; **P<0.01.