Table 2. Glycosaminoglycan synthesis and disaccharide composition of labeled HS isolated from MCF10A, MCF7, MDA-MB-231, and HCC38 cells.
| Cell type | 35S-labeled glycosaminoglycans | |
|---|---|---|
| HS1 % | CS2 % | |
| MCF10A | 58 ± 1 | 42 ± 1 |
| MCF7 | 56 ± 10 | 44 ± 10 |
| MDA-MB-231 | 63 ± 10 | 37 ± 10 |
| HCC38 | 69 ± 5 | 31 ± 10 |
Labeled glycosaminoglycans were extracted from the cell surface and quantitated. The percentage values of HS and CS are given as means from three independent 35S-labeling experiments ± mean deviation.
1
Material resistant to chondroitinase ABC.
2
Material susceptible to chondroitinase ABC.