Figure 8.

(a) Percentage of cells in which the long axis (L) of their nucleus aligned within ±10° of the trench axis when adhered on comb structures with varying trench widths. Results show cell orientation depends strongly on trench width. Three different regimes are observed with widths between (i) 0.21–0.5 μm, (ii) 1–10 μm, and (iii) 50–100 μm; (b) nucleus shape (as defined by the ratio short (S) and long axes (L)) as a function of trench widths. Three different regions can be identified based on the relationships between the length ratios and width of trenches—between (i) 0.21–0.5 μm; (ii) 1–10 μm; and (iii) 50–100 μm.