Figure 9.

(a) Representative 70°-tilted high-magnification SEM micrographs of adherent cells on comb structures with various trench widths. White arrows indicate examples of pseudopodia that bridged across the trenches; (b) schematic illustrations of two spreading behaviors of pseudopodia across trenches—trench bridging and conformal trench coverage; (c) typical 70°-tilted SEM micrographs of cells incubated on parallel trench structures with trench widths of 0.21 and 0.26 μm for 0.5 h. White arrows indicate examples of pseudopodia that bridged across the trenches.