Table 2.
Comparison of biochemical properties of AlyA and partial enzymes of PL7 family.
| Enzyme | Specificity | Km, Vmax | Optimal Temperature and pH | Products (Dp) | Reference |
|---|---|---|---|---|---|
| AlyA | Bifunctional | 5.6 mM, 3.22 nmol/s | 55 °C, 7.5 | 2–5 | This study |
| Algb | polyG > polyM | 0.67 mg/mL, 473.93 U/mg | 30 °C, 8.0 | 2–5 | [17] |
| FsAlgA | polyG > polyM | 0.48 mM, 0.19 nmol/s | 50 °C, 7.0 | 2–5 | [41] |
| Alg7D | polyM > polyG | 3.0 mg/mL, 6.2 U/mg | 50 °C, 7.0 | 2–5 | [13] |
| AlgMsp | polyG > polyM | 3.4 mM, 57 pmol/s | 50 °C, 8.0 | 2–5 | [38] |
| AlgNJ-04 | polyG > polyM | 0.49 mM, 72 pmol/s | 40 °C, 7.0 | 2–5 | [41] |
| AlgNJU-03 | polyG > polyM | 8.5 mM, 1.67 nmol/s | 30 °C, 7.0 | 2–4 | [40] |
| AlgC-PL7 | Bifunctional | - | 55 °C, 8.0 | 1 | [45] |
| A9m | polyG > polyM | - | 30 °C, 7.5 | - | [44] |