Table 1.
Deduced functions of open reading frames (ORFs) in jomthonic acids biosynthetic gene cluster.
| Gene | Deduced aa. Length | Protein Homologue (Accession No.) | Identity/Similarity (%) | Proposed Function |
|---|---|---|---|---|
| orf1 | 194 | WP_030077520.1 | 93/86 | RNA polymerase |
| orf2 | 313 | WP_053208602.1 | 95/96 | Hypothetical protein |
| orf3 | 1462 | WP_084771961.1 | 94/96 | Cell division protein FtsK |
| orf4 | 1525 | WP_055485092.1 | 59/70 | Type IV secretion protein Rhs |
| orf5 | 241 | WP_084771964.1 | 93/95 | Hypothetical protein |
| orf6 | 104 | WP_084771965.1 | 86/94 | Hypothetical protein |
| orf7 | 99 | WP_033268020.1 | 99/100 | WXG-like protein |
| orf8 | 289 | SDQ83237.1 | 89/91 | AraC-family transcriptional regulator |
| orf9 | 396 | SDQ83203.1 | 87/93 | Monooxygenase |
| orf10 | 315 | OSY47464.1 | 92/94 | FG-GAP repeat protein |
| orf11 | 95 | OSY47461.1 | 96/96 | Type II toxin–antitoxin system |
| orf12 | 286 | OSY47460.1 | 91/94 | Thioesterase |
| orf13 | 218 | WP_085923163.1 | 99/99 | ATP-dependent Clp protease proteolytic subunit |
| orf14 | 106 | OSY47458.1 | 95/97 | Hypothetical protein |
| orf15 | - | - | Unassigned | |
| jomP1 | 2093 | WP_020735335.1 | 47/58 | Type I polyketide synthase (PCP/KS/AT/AT/KR/PCP) |
| jomP2 | 1853 | CAD19087.1 | 43/57 | Type I polyketide synthase (KS/AT/DH/KR/PCP) |
| jomN | 1062 | SCL52665.1 | 48/63 | Non-ribosomal protein synthetase(C/A/PCP) |
| jomT | 254 | WP_025675064.1 | 35/51 | Thioesterase |
| jomB | 72 | WP_004933768.1 | 72/83 | mbtH-like protein |
| jomM | 329 | WP_004933770.1 | 78/85 | Methyltransferase |
| orf16 | 751 | WP_053684324.1 | 53/65 | LuxR-family transcriptional regulator |
| orf17 | 229 | WP_042159102.1 | 83/88 | Hypothetical protein |
| orf18 | 404 | WP_052718894.1 | 90/93 | Hypothetical protein |
| orf19 | 295 | SCK58100.1 | 86/91 | Putative nuclease |
| orf20 | 170 | WP_035838695.1 | 81/85 | MarR-family transcriptional regulator |
| orf21 | 123 | WP_039639044.1 | 93/95 | Glyoxalase |
| orf22 | 318 | WP_042157776.1 | 78/83 | DNA-3-methyladenine glycosylase 2 family protein |
| rf23 | 190 | WP_042159103.1 | 97/99 | Hypotheticalprotein |
| orf24 | 373 | WP_042159104.1 | 99/100 | ROK-familyglucokinase |
| orf25 | 269 | WP_085927837.1 | 100/100 | Sugar ABC transporter ATP-binding protein |
| orf26 | 356 | WP_085927838.1 | 99/99 | Ribose ABC transporter permease |
| orf27 | 337 | WP_042159107.1 | 99/100 | Sugar ABC transporter substrate-binding protein |
| orf28 | 358 | WP_042159108.1 | 96/97 | Sugar ABC transporter substrate-binding protein |
| orf29 | 258 | GAO11207.1 | 98/99 | GntR-family transcriptional regulator |
| orf30 | 126 | WP_018092820.1 | 84/90 | Glyoxalase |
| orf31 | 275 | WP_042159111.1 | 95/96 | Putative aminoacid ABC transporter |
| orf32 | 219 | WP_085927844.1 | 99/99 | 2-dehydro-3-deoxy-phosphogluconate aldolase |