Skip to main content
. 2018 Jul 31;34:61–75. doi: 10.1016/j.ebiom.2018.07.013

Fig. 2.

Fig. 2

Changes in mRNA and protein levels in CML blast cells after chidamide treatment. A & B. The mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2, MECP2, and HDAC1 after the KCL22 and K562 cells were treated by 10, 15, and 20 μmol/l chidamide were detected by RT-PCR. C. Chidamide treatment decreased the protein levels of DNMT1, DNMT3A, DNMT3B, MBD2, MECP2, and HDAC1 in KCL22 cells. D. Western blot was used to detect the protein levels of DNMT1, DNMT3A, DNMT3B, MBD2, MECP2, and HDAC1 in KCL22 cells. E. Chidamide treatment decreased the protein levels of these six proteins in K562 cells. F. Western blot was used to detect the protein levels of these six proteins in K562 cells. For Western blot, ACTB was used as a control. For Fig. 2A and B, the one-way ANOVA was used for data analysis. *: P < 0.05 compared to 10 μmol/l group. **: P < 0.01 compared to 10 μmol/l group. #: P < 0.05 compared to 15 μmol/l group. For Fig. 2C and E, the Student's t-test was used for data analysis. **: P < 0.01 compared to control group.