Fig. 5.

MiR-147 overexpression inhibited proliferation, promoted apoptosis, and affected the genes expression. A. The KCL22 cells was transfected with miR-147 control and miR-147 mimics. Compared with the control group, transfection with miR-147 mimics inhibited proliferation (a). The apoptosis rate of the miR-147 mimics group (Q2 + Q4 = 59.19%) was higher than that of the control group (Q2 + Q4 = 11.94%) (b and c). B. The K562 cells were transfected with miR-147-control and miR-147 mimics. Compared with the control group, transfection with the miR-147 mimics inhibited proliferation (a). The apoptosis rate of the miR-147 mimics group (Q2 + Q4 = 51.98%) was higher than that of the control group (Q2 + Q4 = 10.02%) (b and c). C. In the KCL22 cells, the mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2, MECP2, and HDAC1 decreased in the miR-147 mimics group (a); and the DNMT1, DNMT3A, DNMT3B, MBD2, MECP2, and HDAC1 protein levels decreased in the miR-147 mimics group (b and c). D. In the K562 cells, the mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2, MECP2, and HDAC1 decreased in the miR-147 mimics group (a); and the DNMT1, DNMT3A, DNMT3B, MBD2, MECP2, and HDAC1 protein levels decreased in the miR-147 mimics group (b and c). Student's t-test was used for data analysis. The comparison of cell apoptosis rate between miR-147 control group and miR-147 mimic group was analyzed using chi-squared test. *: P < 0.05 compared to miR-147 control group. **: P < 0.01 compared to miR-147 control group.