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. 2018 Jul 31;34:61–75. doi: 10.1016/j.ebiom.2018.07.013

Table 3.

Primer sequences for RT-PCR.

Gene Primer Product
MEG3 Forward: 5′-CTCCCCTTCTAGCGCTCACG-3′ 154 bp
Reverse: 5′-CTAGCCGCCGTCTATACTACCGGCT-3′
DNMT1 Forward: 5′-AGACACTCGCTCAGCTTCTTG-3′ 116 bp
Reverse: 5′-CAATTGCTGCTGGGATTCATC-3′
DNMT3A Forward: 5′-CTGCTTTGTATTCCCTTTTGCA-3′ 141 bp
Reverse: 5′-TTGATTTCTCCTGGCTGTCTC-3′
HDAC1 Forward: 5′-CCTCCCGAGTGAAGTCATCGTGG-3′ 181 bp
Reverse: 5′-GGACAGGTGCTTCATCAGCTCG-3′
DNMT3B Forward: 5′-ACCACACGTTCCTAAGCTGG-3′ 124 bp
Reverse: 5′-TCCCTGCACGCAGAGATTTT-3′
MECP2 Forward: 5′-AACTACAACTTCTTCCCTCGCAA-3′ 109 bp
Reverse: 5′-CAAAGTTATGTCCACTGTCTCT-3′
MBD2 Forward: 5-CATCACCGTAGTCCCATTG-3′ 175 bp
Reverse: 5-AAATTCCCCACTTCGCTTGG-3′
JAK2 Forward: 5′-CCACCCTTGTGCTCTTCCCTG-3′ 100 bp
Reverse: 5′-TCTGCCACCCGAGTGTAACCA-3′
STAT3 Forward:5′-TCCCTGCACGCAGAGATTTT-3′ 131 bp
Reverse: 5′-TTGATTTCTCCTGGCTGTCTC-3′
STAT5 Forward: 5′-AACCCTGCTGTTTGGCTTAAC-3′ 127 bp
Reverse: 5′-CGTACTTGCTGTACTCGCTCTTC-3′
ACTB Forward: 5′-GAGCTACGAGCTGCCTGAC-3′ 121 bp
Reverse: 5′-GGTAGTTTCGTGGATGCCACAG-3′
miR-147 5′-CCCCTATCACGATTAGCATTAA-3′ 82 bp
U6 5′-GCAGGGGCCATGCTAATCTTCTCTGTATCG-3′ 74 bp