Table 1.
Terminal Body Weight, Relative Liver Weights and Clinical Chemistries Following 7 and 28 Days of Exposure to Androstenedione (A4), Ethinyl Estradiol (EE), Phenobarbital (PB) and Prednisone (PRED).
| Terminal Body Weight gm |
Relative Liver Weight % |
ALTb IU/L |
SDHc IU/L |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Day | Chemical | Dosea | n |
LS-Mean (SE) |
n |
LS-Mean (SE) |
n |
LS-Mean (SE) |
n |
LS-Mean (SE) |
| 7 | A41 | 0 | 11 | 31.30 (0.79) |
11 | 4.17 (0.11) |
6 | 52.57 (28.95) |
6 | 19.54 (12.54) |
| 2 | 13 | 31.80 (0.72) |
13 | 4.24 (0.10) |
7 | 55.21 (27.08) |
8 | 36.05 (10.91) |
||
| 10 | 15 | 31.09 (0.67) |
15 | 4.22 (0.09) |
10 | 80.49 (22.36) |
10 | 39.02 (9.68) |
||
| 20 | 15 | 30.62 (0.67) |
15 | 4.38 (0.09) |
9 | 44.17 (23.84) |
9 | 15.82 (10.33) |
||
| 50 | 15 | 31.99 (0.67) |
15 | 4.26 (0.09) |
10 | 37.20 (22.36) |
10 | 17.57 (9.68) |
||
| EE | 0 | 16 | 33.25 (0.52) |
16 | 4.48 (0.36) |
9 | 56.99 (73.94) |
10 | 25.97 (24.37) |
|
| 1 | 16 | 24.38*** (0.52) |
16 | 4.56 (0.36) |
10 | 52.96 (70.22) |
10 | 20.78 (24.37) |
||
| 2.5 | 16 | 24.28*** (0.52) |
16 | 4.48 (0.36) |
10 | 87.75 (70.22) |
9 | 14.84 (25.94) |
||
| 5 | 16 | 24.09*** (0.52) |
16 | 4.66 (0.36) |
11 | 321.32 (66.87) |
10 | 68.91 (24.37) |
||
| 10 | 16 | 25.04*** (0.52) |
16 | 4.81 (0.36) |
8 | 29.82 (78.95) |
8 | 65.05 (27.13) |
||
| PB | 0 | 16 | 33.25 (0.52) |
16 | 4.48 (0.10) |
9 | 69.69 (26.15) |
10 | 30.70 (7.14) |
|
| 600 | 16 | 32.43 (0.52) |
16 | 6.03*** (0.10) |
10 | 105.73 (24.86) |
10 | 33.19 (7.14) |
||
| PRED | 0 | 16 | 33.25 (0.53) |
16 | 4.48 (0.09) |
9 | 69.99 (13.76) |
10 | 30.86 (5.17) |
|
| 1.25 | 16 | 31.94 (0.53) |
16 | 4.05** (0.09) |
9 | 68.77 (13.77) |
10 | 24.29 (5.17) |
||
| 2.5 | 16 | 32.44 (0.53) |
16 | 4.22 (0.09) |
10 | 58.92 (13.07) |
10 | 23.95 (5.17) |
||
| 5 | 16 | 30.31*** (0.53) |
16 | 4.18 (0.09) |
10 | 70.47 (13.07) |
10 | 23.33 (5.17) |
||
| 25 | 16 | 29.62*** (0.54) |
16 | 4.55 (0.09) |
10 | 92.01 (13.07) |
10 | 35.85 (5.17) |
||
| 28 | A42 | 0 | 13 | 34.73 (0.76) |
13 | 4.12 (0.14) |
9 | 37.97 (4.29) |
9 | 28.75 (16.95) |
| 50 | 20 | 34.43 (0.61) |
20 | 4.21 (0.11) |
13 | (37.21) (3.49) |
14 | 45.21 (13.38) |
||
| EE | 0 | 16 | 38.43 (0.61) |
16 | 4.02 (0.13) |
10 | 43.14 (28.34) |
10 | 29.15 (8.47) |
|
| 10 | 21 | 21.77*** (0.54) |
21 | 6.73*** (0.11) |
12 | 125.58** (25.63) |
13 | 41.99 (7.38) |
||
| PB | 0 | 16 | 38.43 (0.70) |
16 | 4.02 (0.21) |
10 | 36.54 (8.08) |
10 | 26.52 (5.02) |
|
| 600 | 183 | 35.81* (0.67) |
18 | 6.17*** (0.20) |
11 | 79.02** (7.65) |
11 | 45.51* (4.75) |
||
| PRED | 0 | 16 | 38.43 (0.69) |
16 | 4.02 (0.11) |
10 | 36.36 (5.13) |
10 | 26.65 (2.83) |
|
| 25 | 194 | 31.45*** (0.64) |
19 | 4.42* (0.10) |
12 | 72.37*** (4.64) |
12 | 33.03 (2.56) |
||
n reduced to less than 16 per dose/group due to deaths over the course of the 7 dosing period.
7 mice in the control group and 4 mice in the 50 mg/kg/day dose group died over the course of 28 day dosing period.
2 mice died over the course of the 28 day dosing period.
1 mouse died over the course of the 28 day dosing period.
Least-squares (LS) means and standard errors (SE) by dose for 7- and 28-day steroid exposed mice.
p < 0.05,
p < 0.01,
p < 0.001.
Dose is the gavage dose in mg chemical/kg body weight for A4, dietary concentration (ppm in diet) for EE and PRED, and ppm in drinking water for PB.
Alanine aminotransferase
Sorbitol dehydrogenase