Fig. 1.

Characterization of SHED isolated from a patient with DS. a The morphology of cells in the Ctrl- and DS-SHED was observed using phase-contrast microscopy. Scale bar = 100 μm. b Chromosome 21 (white arrows) from the Ctrl- and DS-SHED cells was visualized with FISH. Scale bar = 5 μm. c Ctrl- and DS-SHED were stained with anti-STRO-1 (upper panel) and anti-nestin (lower panel) antibodies. The nuclei were counterstained with DAPI. Cells expressing low levels of nestin are indicated with yellow arrows. Scale bar = 50 μm. d Nestin expression in Ctrl- and DS-SHED was analyzed using western immunoblotting. The nestin expression was normalized with HSP90. The mean ± SEM from three independent experiments is shown. *P < 0.05