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. 2018 Aug 31;18:132. doi: 10.1186/s12883-018-1140-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — DN differentiation of DS-SHED. a Ctrl- and DS-DN cells were observed by immunofluorescence microscopy using anti-β-tubulin III (left panel) and anti-TH (right panel) antibodies. The nuclei were counterstained with DAPI. Scale bar = 50 μm. b, c Neurite length (b) and number of branches (c) of Ctrl- and DS-DN cells were measured. The mean ± SEM from 100 cells is shown. ***P < 0.001. d DN development was classified into 4 stages. The upper panel shows original TH immunofluorescence images, and the lower panel shows the output from Neurite Outgrowth module of MetaMorph software. e A total of 100 differentiated DN were categorized into 4 stages and shown on the graph