Fig. 3.

CHI3L1 triggers Erk and Akt signaling through CD44. a and b Western blot analysis of Erk and Akt activation in the AGS and MGC803 cells which were pre-exposed with the control antibody or functional CD44 blocking antibody and treated with rhCHI3L1 (500 ng/ml) for the indicated times. c and d Western blot analysis of CD44 and IL-13Rα2 knockdown efficacy in AGS (c) and MGC803 (d) transfected with scramble, CD44 or IL-13Rα2 shRNA. e and f Westen blot evaluation of the activation of Erk and Akt in AGS and MGC803 cells which stably expressing scrambled, CD44 or IL-13Rα2 shRNA and were cultured in the presence or absence of rhCHI3L1 (500 ng/ml). g Westen blot analysis of the activation of Erk and Akt in bone marrow-derived macrophages (BMDM) from WT and CD44^−/− mice which were incubated with the recombinant mouse CHI3L1 (500 ng/ml) for the noted periods of time