Fig. 5.

Roles of CHI3L1 in promoting GC growth and metastasis in vitro. a The effects of rhCHI3L1 on AGS and MGC803 cells proliferation were measured by CCK8 assay. Data are presented as mean ± SEM. b Colony formation assays were performed for AGS and SGC7901 cells. c and d Proliferation of AGS (c) and MGC803 (d) cells were evaluated by EdU incorporation assay. e The invasion assay of AGS and MGC803 cells. f AGS cells transfected with scramble control or shRNAs targeting CD44 were subjected to wound closure assays. g GC cells were incubated with rhCHI3L1for the noted periods of time. Protein levels of Vimentin and Snail were analyzed by Western blot. CHI3L1 (500 ng/ml) in combination with control or functional CD44 neutralizing antibody (10 μg/ml) were used in the experiments. Results shown here are the representative of three independent experiments. Scale bars represent 100 μm. Statistical significance was calculated using ANOVA (a-f). *p < 0.05