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. 2018 Aug 31;18:133. doi: 10.1186/s12883-018-1139-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Overexpressed miR-139-5p inhibited glioma metastasis, invasion and EMT. (a and b) Wound healing assays confirmed that miR-139-5p suppressed the migration of LN229 and U87 cells. (× 50 magnification). (c and d) Transwell assays indicated that miR-139-5p inhibited LN229 and U87 cell invasion. (× 50 magnification). (e and f) The mRNA expression levels of different genes. The qRT-PCR analysis demonstrated that the upregulation of miR-139-5p resulted in a reduction of Notch1, N-cadherin, Vimentin and Fibronectin mRNA expression and an elevation of E-cadherin mRNA expression. (g and h) The Western blot analysis showed that the protein expression of Notch1, N-cadherin, Vimentin and Fibronectin decreased, and the protein expression of E-cadherin increased in LN229 and U87 cells after transfection with miR-139-5p. (i) Immunofluorescence showed miR-139-5p overexpression increased E-cadherin expression and decreased Vimentin expression in glioma cells. This experiment was repeated three times. (× 50 magnification). (*P < 0.05. **P < 0.01. ***P < 0.001)