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. 2018 Aug 29;475(16):2577–2592. doi: 10.1042/BCJ20160866

Figure 1. Autoclaved yeast media contains traces of pyruvate, formed non-enzymatically in the heat sterilisation procedure.

Figure 1.

(Left-hand side) Pathway map of glycolysis and the PPP as occurring in Saccharomyces cerevisiae. Modified from [47]. (Right-hand side) Selective reaction monitoring, as conducted in [47], detects pyruvate in yeast synthetic complete media (SC) upon autoclaving it at 121°C for 20 minutes (upper panel).