Figure 1. Autoclaved yeast media contains traces of pyruvate, formed non-enzymatically in the heat sterilisation procedure.
(Left-hand side) Pathway map of glycolysis and the PPP as occurring in Saccharomyces cerevisiae. Modified from [47]. (Right-hand side) Selective reaction monitoring, as conducted in [47], detects pyruvate in yeast synthetic complete media (SC) upon autoclaving it at 121°C for 20 minutes (upper panel).