Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 29;475(16):2593–2610. doi: 10.1042/BCJ20180232

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Author(s)

This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).

PMC Copyright notice

Figure 1. — (A) Purity of recombinant enzyme by SDS–PAGE: insoluble (lane 1), soluble (lane 2), pooled fraction after HisTrap (lane 3) and pooled protein after hexahistidine tag removal (lane 4). (B) Elution profile of recombinant TcAKR (black) and His₆-TcAKR (blue) under quasi-physiological conditions from a Superdex 75 10/300 column. (Inset) Elution volumes of known protein standards (open circles) plotted against the log₁₀ of their molecular masses. Theoretical elution volume of the T. cruzi AKR monomer (closed circle). Linear fit R²: 0.998. (C) Elution profile of recombinant TcAKR at 2 mg ml⁻¹ (black) and 20 mg ml⁻¹ (blue) under quasi-physiological conditions from a Superose12 10/300 column.