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. 2018 Aug 29;475(16):2593–2610. doi: 10.1042/BCJ20180232

Table 2. MRM transitions used for detecting the prostaglandin species and their retention times.

Chromatograms of the 10 min chromatographic separations are provided in Supplementary Figure S1.

Prostaglandin MRM transition Retention time (min)
6-keto PGF 369.2 > 163.1 3.09
Iso-PGF 353.0 > 192.9 4.25
PGF 353.0 > 192.9 4.88
PGF 355.2 > 311.2 4.96
PGE2 351.1 > 271.1 5.06
PGE1 353.2 > 317.2 5.31