Table 3. A survey of purified recombinant trypanosomatid aldo-keto reductases with various substrates.

Compounds (60 µM) were assayed spectrophotometrically in the presence of 100 µM NADPH. Specific activity is given in µmol min⁻¹ mg⁻¹. Values are the means and standard deviations (n = 3).

Substrate	TcAKR	LiPGFS1	LiPGFS2	TbPGFS
2-Nitrobenzaldehyde	0.20 ± 0.004	1.40 ± 0.04	2.51 ± 0.029	0.73 ± 0.017
3-Nitrobenzaldehyde	0.16 ± 0.003	0.06 ± 0.002	2.40 ± 0.037	1.86 ± 0.009
4-Nitrobenzaldehyde	0.39 ± 0.009	0.06 ± 0.002	2.49 ± 0.142	0.71 ± 0.013
Glyoxal	0.001 ± 0.001	0.02 ± 0.001	0.09 ± 0.001	0.15 ± 0.005
Methylglyoxal	1.20 ± 0.022	0.61 ± 0.02	1.79 ± 0.009	0.67 ± 0.012
Phenylglyoxal	3.66 ± 0.092	3.11 ± 0.018	2.46 ± 0.084	0.78 ± 0.037
Vitamin K3 epoxide	N.A.	N.A.	N.D.	N.A.
Glyceraldehyde	0.05 ± 0.005	N.D.	0.16 ± 0.002	0.13 ± 0.004
Benznidazole	N.A.	N.A.	N.A.	N.A.

Abbreviations: NA, not active; ND, not determined.