Table 5. Kinetic characterisation of trypanosomatid recombinant aldo-keto reductases with various substrates.

Substrate	Units	TcAKR	LiPGFS1	LiPGFS2	TbPGFS
Glyoxal
kcat	s−1	4.56 ± 0.05	7.31 ± 0.19	1.05 ± 0.02	ND
$K_{\mathrm{m}}^{\mathrm{app}}$1	mM	8.30 ± 0.31	36.1 ± 2.39	0.56 ± 0.05	ND
$k_{\mathrm{cat}}/K_{\mathrm{m}}^{\mathrm{app}}$	M−1 s−1	549	202	1880	ND
Methylglyoxal
kcat	s−1	4.69 ± 0.03	8.72 ± 0.17	1.13 ± <0.01	0.38 ± 0.007
$K_{\mathrm{m}}^{\mathrm{app}}$	mM	0.24 ± <0.001	0.80 ± 0.07	0.02 ± <0.001	0.01 ± <0.001
$k_{\mathrm{cat}}/K_{\mathrm{m}}^{\mathrm{app}}$	M−1 s−1	19 500	10 900	56 500	38 000
Phenylglyoxal
kcat	s−1	4.46 ± 0.08	10.4 ± 0.22	1.17 ± 0.02	0.34 ± 0.014
$K_{\mathrm{m}}^{\mathrm{app}}$	mM	0.05 ± 0.003	0.14 ± 0.008	0.01 ± 0.0009	0.005 ± 0.0008
$k_{\mathrm{cat}}/K_{\mathrm{m}}^{\mathrm{app}}$	M−1 s−1	89 200	74 300	58 500	68 000
2-Nitrobenzaldehyde
kcat	s−1	2.25 ± 0.112	8.50 ± 0.21	1.04 ± 0.02	0.41 ± 0.002
$K_{\mathrm{m}}^{\mathrm{app}}$	mM	0.71 ± 0.082	0.32 ± 0.02	0.0024 ± 0.0003	0.003 ± 0.0003
$k_{\mathrm{cat}}/K_{\mathrm{m}}^{\mathrm{app}}$	M−1 s−1	3170	26 600	433 000	140 000
3-Nitrobenzaldehyde
kcat	s−1	4.07 ± 0.17	1.28 ± 0.03	1.04 ± 0.02	0.34 ± 0.005
$K_{\mathrm{m}}^{\mathrm{app}}$	mM	2.20 ± 0.16	1.55 ± 0.06	0.002 ± 0.0003	0.002 ± 0.0001
$k_{\mathrm{cat}}/K_{\mathrm{m}}^{\mathrm{app}}$	M−1 s−1	1850	830	520 000	170 000
4-Nitrobenzaldehyde
kcat	s−1	3.7 ± 0.15	0.48 ± 0.01	1.13 ± 0.052	0.43 ± 0.008
$K_{\mathrm{m}}^{\mathrm{app}}$	mM	68 ± 0.07	0.43 ± 0.026	0.004 ± 0.00062	0.0043 ± 0.0002
$k_{\mathrm{cat}}/K_{\mathrm{m}}^{\mathrm{app}}$	M−1 s−1	5440	1 110	283 000	100 000
d,l-glyceraldehyde
kcat	s−1	4.94 ± 0.11	8.72 ± 0.112	2.46 ± 0.132	0.37 ± 0.009
$K_{\mathrm{m}}^{\mathrm{app}}$	mM	1.50 ± 0.17	2.87 ± 0.082	1.07 ± 0.152	0.07 ± 0.005
$k_{\mathrm{cat}}/K_{\mathrm{m}}^{\mathrm{app}}$	M−1 s−1	3290	3040	2300	5290

Abbreviations: ND, not determined.

¹$K_{\mathrm{m}}^{\mathrm{app}}$ values determined with 100 µM NADPH.

²Weighted mean of two individual replicates, all others are n ≥ 3.