Fig. 4.
Receptor-mediated transcytosis of antibodies across the BBB on-a-chip model. a 3D artist impression of the assessment of antibody transcytosis across BBB co-cultures in the three-lane OrganoPlate. Antibody is perfused through the lumen of the TY10 microvessel. Samples are taken from the apical compartment (ECM gel channel and channel in which hAst and hBPCT cells are grown). b Apical samples taken from BBB co-cultures and TY10-free controls were analyzed and apparent permeability (Papp) of control antibody anti-HEL and target antibody MEM-189 are plotted. n = 2–6 for BBB co-cultures and n = 1–2 for TY10-free controls. Error bars show the standard deviation of the mean