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. 2018 Sep 1;198(5):599–609. doi: 10.1164/rccm.201711-2242OC

Figure 1.

Figure 1.

Stiff matrix upregulates DSP (desmoplakin) expression in lung epithelial cells. Human and rodent lung epithelial cells were cultured on soft and stiff polyacrylamide gels for 48 hours. (A) Relative mRNA levels of DSP were determined by quantitative PCR (qPCR). (B) Protein levels of DSP were determined by immunoblot. GAPDH was used as reference/loading control. Results are mean ± SD of five independent experiments, each qPCR reaction performed in triplicate. *P < 0.05. A549 = human alveolar epithelial adenocarcinoma cells; mAECII = mouse type II alveolar epithelial cells; MLE = mouse lung epithelial cells; RLE-6TN = transformed rat type II alveolar epithelial cells.