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. 2018 Aug 15;198(4):486–496. doi: 10.1164/rccm.201709-1823OC

Figure 4.

Figure 4.

Enforced YAP (yes-associated protein) expression does not restore mature miR-29 (microRNA-29) expression on idiopathic pulmonary fibrosis (IPF)–extracellular matrix (ECM). (A–D) Fibroblasts were transduced with empty vector, YAP S127/381A–FLAG-tagged, or YAP 5SA–MYC-tagged and cultured on IPF-ECM for 18 hours. (A) Ectopic YAP expression was analyzed by immunoblot for anti-FLAG and anti-MYC. (B) YAP target genes CTGF and CYR61 were quantified by quantitative PCR (qPCR) normalized to GAPDH. (C) Primary–precursor miR-29a and -29c were quantified by qPCR normalized to GAPDH; (D) mature miR-29a, -29b, and -29c were quantified by qPCR normalized to RNU6 (n = 2, representative experiment shown). Error bars represent means ± SD for B and C, and a box-and-whisker plot is shown for D. P value was calculated using a one-way ANOVA test followed by a Tukey test. *P < 0.001, **P < 0.0001.