Table 1.
ADAM33 gene SNP genotyping by PCR-RFLP: primers, restriction enzymes, and length of the digested product fragments
| ADAM33 SNP | Primer sequences |
Restriction
enzyme |
Recognition site | Fragment size (bp) |
|---|---|---|---|---|
| T+1 (rs2280089) (intron 20) |
F: AGGGTCTGGGAGAAATGGTG R: TCTTTGGAAGCTGAGCGATG |
MboII | …GAAGA(N)8↓… …CTTCT(N)7↑… |
GG: 424+132 bp AG: 424+132+123+301 bp AA: 301+132+123 bp |
| T1 (rs2280091) (exon 20) |
F: GTGAATATGGTCAGCAGGAG R: GTGACTTGGAGCAGATGG |
NcoI | …C↓CATGG… …GGTAC↑C… |
GG: 375 bp GA: 375+187+188 bp AA: 187+188 bp |
| S1 (rs3918396) (exon 19) |
F: GTGGCAGCATGGACAGT R: CAGGAGTAGGCTCAGGAAG |
BtsCI | …GGATGNN↓… …CCTAC↑NN… |
GG: 304 bp GA: 304+153+151 bp AA: 151+153 bp |
| F+1 (rs511898) (intron 6) |
F: AAATACGACTCGAGGC R: GGACTTCTCAACCCACGAG |
BsmBI | …CGTCTC(N)1↓… …GCAGAG(N)5↑… |
TT: 220 bp TC: 220+183+37 bp CC: 183+37 bp |